Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C′ fragment complementation system
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منابع مشابه
Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C' fragment complementation system.
Escherichia coli thioredoxin was cleaved with CNBr at its single Met residue at position 37, which lies in the middle of a long alpha-helix. The two fragments, 1-37 and 38-108, were purified and characterized by using CD and fluorescence spectroscopy. Both fragments lack structure at neutral pH and room temperature. The secondary and tertiary structural contents of the non-covalent complex form...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1999
ISSN: 0264-6021
DOI: 10.1042/0264-6021:3390721